Lesion processing: high-fidelity versus lesion-bypass DNA polymerases
نویسندگان
چکیده
منابع مشابه
Lesion bypass DNA polymerases replicate across non-DNA segments.
A critical feature of the robustness of the DNA replication machinery is the ability to complete its task in the presence of interfering DNA damage. A key mechanism responsible for this task is translesion replication (also termed translesion synthesis), carried out by specialized lesion bypass DNA polymerases of the Y superfamily. Here we show that in Escherichia coli, plasmids can be replicat...
متن کاملAccuracy, lesion bypass, strand displacement and translocation by DNA polymerases.
The structures of DNA polymerases from different families show common features and significant differences that shed light on the ability of these enzymes to accurately copy DNA and translocate. The structure of a B family DNA polymerase from phage RB69 exhibits an active-site closing conformational change in the fingers domain upon forming a ternary complex with primer template in deoxynucleos...
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The enzyme ribonucleotide reductase, responsible for the synthesis of deoxyribonucleotides (dNTP), is upregulated in response to DNA damage in all organisms. In Saccharomyces cerevisiae, dNTP concentration increases approximately 6- to 8-fold in response to DNA damage. This concentration increase is associated with improved tolerance of DNA damage, suggesting that translesion DNA synthesis is m...
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DNA polymerase (Pol ) is a newly discovered member of the polymerase X family with unknown cellular function. The understanding of Pol function should be facilitated by an understanding of its biochemical activities. By using purified human Pol for biochemical analyses, we discovered the lesion bypass activities of this polymerase in response to several types of DNA damage. When it encountered ...
متن کاملTrans-Lesion DNA Polymerases May Be Involved in Yeast Meiosis
Trans-lesion DNA polymerases (TLSPs) enable bypass of DNA lesions during replication and are also induced under stress conditions. Being only weakly dependent on their template during replication, TLSPs introduce mutations into DNA. The low processivity of these enzymes ensures that they fall off their template after a few bases are synthesized and are then replaced by the more accurate replica...
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ژورنال
عنوان ژورنال: Trends in Biochemical Sciences
سال: 2008
ISSN: 0968-0004
DOI: 10.1016/j.tibs.2008.02.004